Research projects
1) Neuroproteomics: Description of the synaptic proteome and its plasticity
- Changes in the synaptic proteome due to sleep deprivation
- Characterizing of post translational modifications (glycosylation and phosphorylation) present in the synaptic proteome
2) Proteomics and glycoproteomics: Biochemical and clinical applications
- Characterization of protein mixtures, body fluid and tissue proteomics. Protein sequencing and detection of native protein complexes. Development of novel experimental methodologies for proteomics
- Glycobiology, analytical characterization of protein glycosylation, and changes in the glycosylation pattern; with focus on site specific N-glycosylation. Changes of glycosylation in pathological processes, development and application of glycosylation based tumor markers for cancer diagnosis
- Proteomics of extracellular vesicles and exosomes
3) Basic research and (bio)informatics
- Development and application of bioinformatics for evaluation of experimental results in biochemical and clinical proteomics (GlycoMiner & GlycoPattern)
- Description fundamentals of tandem mass spectrometry; the role of energetics (MassKinetics)
Equipment
Bruker Maxis II ETD Q-TOF mass spectrometer coupled to Thermo Dionex UltiMate 3000 nanoUPLC
Waters Q-TOF Premier mass spectrometer coupled to nanoAcquity UHPLC
Waters Micromass Quattro-micro mass spectrometer coupled to Acquity UPLC
Cooperation
The MS Proteomics Research group has various collaborations with universities and research groups in Hungary and abroad. We are also doing collaborative and contract research (structure analysis, qualitative and quantitative analysis) for various companies.
Recent Publications
- Toth Gabor, Vekey Karoly, Drahos Laszlo, Horvath Viola, Turiak Lilla:
Salt and solvent effects in the microscale chromatographic separation of heparan sulfate disaccharides
JOURNAL OF CHROMATOGRAPHY A 1610: Article number 460548 (2020)
10.1016/j.chroma.2019.460548 - Turiak Lilla, Ozohanics Oliver, Toth Gabor, Acs Andras, Revesz Agnes, Vekey Karoly, Telekes Andras, Drahos Laszlo:
High sensitivity proteomics of prostate cancer tissue microarrays to discriminate between healthy and cancerous tissue
JOURNAL OF PROTEOMICS 197: pp. 82-91 (2019)
10.1016/j.jprot.2018.11.009 - Turiak Lilla, Sugar Simon, Acs Andras, Toth Gabor, Gomory Agnes, Telekes Andras, Vekey Karoly, Drahos Laszlo:
Site-specific N-glycosylation of HeLa cell glycoproteins
SCIENTIFIC REPORTS 9: Article number 14822 (2019)
10.1038/s41598-019-51428-x - Sugar Simon, Turiak Lilla, Vekey Karoly, Drahos Laszlo:
Widespread presence of bovine proteins in human cell lines
JOURNAL OF MASS SPECTROMETRY (2019)
10.1002/jms.4464 - Revesz Agnes, Rokob Tibor Andras, Fouque, Dany Jeanne Dit, Huse Daniel, Hada Viktor, Turiak Lilla, Memboeuf Antony, Vekey Karoly, Drahos Laszlo:
Optimal Collision Energies and Bioinformatics Tools for Efficient Bottom-up Sequence Validation of Monoclonal Antibodies
ANALYTICAL CHEMISTRY 91: (20) pp.13128-13135 (2019)
10.1021/acs.analchem.9b03362 - Acs Andras, Turiak Lilla, Revesz Agnes, Vekey Karoly, Drahos Laszlo:
Identification of bifucosylated glycoforms using low-energy CID spectra
JOURNAL OF MASS SPECTROMETRY (2019)
10.1002/jms.4432 - Turiak Lilla, Kaszas Balint, Katona Krisztian, Lacza Agnes, Mark Laszlo, Vekey Karoly, Drahos Laszlo, Tornoczky Tamas:
Localized Amyloidosis of the Upper Aerodigestive Tract: Complex Analysis of the Cellular Infiltrate and the Amyloid Mass
ANALYTICAL CELLULAR PATHOLOGY Article number 6165140 (2019)
10.1155/2019/6165140 - Pocsfalvi Gabriella, Turiak Lilla, Ambrosone Alfredo, del Gaudio Pasquale, Puska Gina, Fiume Immacolata, Silvestre Teresa, Vekey Karoly:
Physiochemical and protein datasets related to citrus juice sac cells-derived nanovesicles and microvesicles
DATA IN BRIEF 22: pp. 251-254 (2019)
10.1016/j.dib.2018.12.036 - Toth Gabor, Panic-Jankovic Tanja, Mitulovic Goran:
Pillar array columns for peptide separations in nanoscale reversed-phase chromatography
JOURNAL OF CHROMATOGRAPHY A 1603: pp. 426-432 (2019)
10.1016/j.chroma.2019.06.067 - Pocsfalvi Gabriella, Turiak Lilla, Ambrosone Alfredo, del Gaudio Pasquale, Puska Gina, Fiume Immacolata, Silvestre Teresa, Vekey Karoly:
Protein biocargo of citrus fruit-derived vesicles reveals heterogeneous transport and extracellular vesicle populations
JOURNAL OF PLANT PHYSIOLOGY 229: pp. 111-121 (2018)
10.1016/j.jplph.2018.07.006 - Revesz, Agnes, Rokob, Tibor Andras, Fouque, Dany Jeanne Dit, Turiak, Lilla, Membouf, Antony, Vekey, Karoly, Drahos, Laszlo:
Selection of Collision Energies in Proteomics Mass Spectrometry Experiments for Best Peptide Identification: Study of Mascot Score Energy Dependence Reveals Double Optimum
JOURNAL OF PROTEOME RESEARCH 17: (5) pp. 1898-1906 (2018)
10.1021/acs.jproteome.7b00912 - Acs, Andras, Ozohanics, Oliver, Vekey, Karoly, Drahos, Laszlo, Turiak, Lilla:
Distinguishing Core and Antenna Fucosylated Glycopeptides Based on Low-Energy Tandem Mass Spectra
ANALYTICAL CHEMISTRY 90: (21) pp. 12776-12782 (2018)
10.1021/acs.analchem.8b03140 - Turiak Lilla, Toth Gabor, Ozohanics Oliver, Revesz Agnes, Acs Andras, Vekey Karoly, Zaia Joseph, Drahos Laszlo:
Sensitive method for glycosaminoglycan analysis of tissue sections
JOURNAL OF CHROMATOGRAPHY A 1544: pp. 41-48 (2018)
10.1016/j.chroma.2018.02.034 - Gyorffy Balazs A, Kun Judit, Torok Gyorgy, Bulyaki Eva, Borhegyi Zsolt, Gulyassy Peter, Kis Viktor, Szocsics Peter, Micsonai Andras, Matko Janos, Drahos Laszlo, Juhasz Gabor, Kekesi Katalin A, Kardos Jozsef:
Local apoptotic-like mechanisms underlie complement mediated synaptic pruning
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 115: (24) pp. 6303-6308 (2018)
10.1073/pnas.1722613115 - Nemeth Andrea, Orgovan Norbert, Sodar Barbara W, Osteikoetxea Xabier, Paloczi Krisztina, Szabo-Taylor Katalin E, Vukman Krisztina V, Kittel Agnes, Turiak Lilla, Wiener Zoltan, Toth Sara, Drahos Laszlo, Vekey Karoly, Horvath Robert, Buzas Edit I.:
Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA
SCIENTIFIC REPORTS 7: Article number 8202 (2017)
10.1038/s41598-017-08392-1 - Foldes Tamas, Madarasz Adam, Revesz Agnes, Dobi Zoltan, Varga Szilard, Hamza Andrea, Nagy Peter R, Pihko, Petri M, Papai Imre:
Stereocontrol in Diphenyiprolinol Silyl Ether Catalyzed Michael Additions: Steric Shielding or Curtin-Hammett Scenario?
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 139: (47) pp. 17052-17063 (2017)
10.1021/jacs.7b07097 - Sodar Barbara W, Kovacs Arpad, Visnovitz Tamas, Pallinger Eva, Vekey Karoly, Pocsfalvi Gabriella, Turiak Lilla, Buzas Edit I.:
Best practice of identification and proteomic analysis of extracellular vesicles in human health and disease
EXPERT REVIEW OF PROTEOMICS 14: (12) pp. 1073-1090 (2017)
10.1080/14789450.2017.1392244
Leader
László Drahos